Journal: Biomedicines

Article Title: CB2 Agonist GW842166x Protected against 6-OHDA-Induced Anxiogenic- and Depressive-Related Behaviors in Mice

doi: 10.3390/biomedicines10081776

Figure Lengend Snippet: GW ameliorated the in vivo reduction in spontaneous dopamine transients in the NAc. ( A ) Timeline of intra-striatal injection of 6-OHDA, AAV9-GRABDA2m and AAV8-mCherry, drug treatments, and fiber photometry recording. ( B ) Diagram showing targeting of AAV and cannula implant to the NAc (left) and a representative post hoc validation of AAV expression and optic fiber position (right). ( C ) Schematic demonstrating the path of excitation LEDs and reporter emission signals during fiber photometry recording. ( D ) Left, representative traces of GRABDA2m ΔF/F in the NAc of mice in control and treatment groups. Intra-striatal 6-OHDA injections significantly decreased the number of events/min relative to control injections (*** p < 0.001; n = 6–7 mice). Right, chronic treatment with GW treatment mitigated the decrease in dopamine event frequency by 6-OHDA (** p = 0.001; n = 6–7 mice). The effect of GW was blocked by co-treatment with AM (* p = 0.032; n = 7–7 mice).

Article Snippet: A low autofluorescence fiber-optic cannula (OD = 200 µm, NA = 0.57; Doric Lenses, Inc. Québec, QC, Canada.) was implanted in the striatum above the site of AAV injection.

Techniques: In Vivo, Injection, Expressing

Journal: STAR Protocols

Article Title: Protocol for synchronized wireless fiber photometry and video recordings in rodents during behavior

doi: 10.1016/j.xpro.2024.103407

Figure Lengend Snippet:

Article Snippet: Optic fiber cannula , RWD Life Science Co. , Cat. #R-FOC-BF400C39NA.

Techniques: Virus, Plasmid Preparation, Software, Injection, Ointment